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mir 506 3p mimics  (Addgene inc)


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    Addgene inc mir 506 3p mimics
    Mir 506 3p Mimics, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mir 506 3p mimics/product/Addgene inc
    Average 93 stars, based on 5 article reviews
    mir 506 3p mimics - by Bioz Stars, 2026-02
    93/100 stars

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    MiR-506-3p suppresses the migration and invasion of OvCa cells. SKOV3 cells were transfected with miR-506-3p mimics or miR-ctrl for 48 hours. Transwell assays (A) and wound healing assay (B) were used to assess cell migration and invasion capacities in vitro. (A) Representative images of migrated and invaded SKOV3 cells under the microscope (magnification, x200). The migration and invasion ability of SKOV3 cells after miR-506-3p transfection is lower than that of miR-ctrl transfected cells (** p <0.01). (B) The ability of wound healing was observed under a microscope after 0, 18 hours. The data was analyzed as width ratio (18 hours width/0 hour width). The wound closure rate of SKOV3 cells transfected with miR-506-3p mimics was significantly lower than that of cells transfected with miR-ctrl (** p <0.01).

    Journal: Journal of Cancer

    Article Title: MicroRNA-506-3p inhibits ovarian cancer metastasis by down-regulating the expression of EZH2

    doi: 10.7150/jca.66959

    Figure Lengend Snippet: MiR-506-3p suppresses the migration and invasion of OvCa cells. SKOV3 cells were transfected with miR-506-3p mimics or miR-ctrl for 48 hours. Transwell assays (A) and wound healing assay (B) were used to assess cell migration and invasion capacities in vitro. (A) Representative images of migrated and invaded SKOV3 cells under the microscope (magnification, x200). The migration and invasion ability of SKOV3 cells after miR-506-3p transfection is lower than that of miR-ctrl transfected cells (** p <0.01). (B) The ability of wound healing was observed under a microscope after 0, 18 hours. The data was analyzed as width ratio (18 hours width/0 hour width). The wound closure rate of SKOV3 cells transfected with miR-506-3p mimics was significantly lower than that of cells transfected with miR-ctrl (** p <0.01).

    Article Snippet: According to the manufacturer's instructions, the miR-506-3p mimic or miR-ctrl was transfected into the OvCa cells at a final concentration of 20 nM with LipofectamineRNAiMAX (Invitrogen, USA).

    Techniques: Migration, Transfection, Wound Healing Assay, In Vitro, Microscopy

    EZH2 and miR-506-3p induced decrease in migration and invasion of OvCa cells. SKOV3 cells were co-transfected with empty vector (EV) or EZH2 without the 3'-UTR together with 20 nM miR-ctrl or miR-506-3p. After 24 hours, the cells were harvested for western blot analysis (A) or reseeded for Transwell assay (B).

    Journal: Journal of Cancer

    Article Title: MicroRNA-506-3p inhibits ovarian cancer metastasis by down-regulating the expression of EZH2

    doi: 10.7150/jca.66959

    Figure Lengend Snippet: EZH2 and miR-506-3p induced decrease in migration and invasion of OvCa cells. SKOV3 cells were co-transfected with empty vector (EV) or EZH2 without the 3'-UTR together with 20 nM miR-ctrl or miR-506-3p. After 24 hours, the cells were harvested for western blot analysis (A) or reseeded for Transwell assay (B).

    Article Snippet: According to the manufacturer's instructions, the miR-506-3p mimic or miR-ctrl was transfected into the OvCa cells at a final concentration of 20 nM with LipofectamineRNAiMAX (Invitrogen, USA).

    Techniques: Migration, Transfection, Plasmid Preparation, Western Blot, Transwell Assay

    MiR-506-3p suppresses OvCa tumor metastasis of an orthotopic mouse model of ovarian cancer. HeyA8 cells were injected intraperitoneally into a nu/nu mouse, and an aggressive HeyA8 clone generated from ascites was developed for subsequent use (HeyA8ip1). The mice were randomly divided into control miRNA+DOPC and miR-506-3p+DOPC groups after intraperitoneal injection of HeyA8ip1 cells. (A) In vivo imaging confirmed that miR-506/DOPC nanoliposomes can inhibit OvCa tumor metastasis. (B) Comparison of the incidence of metastases in various organs. (C) Evaluation of miR-506-3p expression in HeyA8-ip1 tumors from control and miR-506-3p treated mice by miRNA in situ hybridization. EZH2 IHC staining was performed on HeyA8-ip1 tumor samples from control and miR-506-3p treated mice. The expression of EZH2 was calculated as the IHC staining score.

    Journal: Journal of Cancer

    Article Title: MicroRNA-506-3p inhibits ovarian cancer metastasis by down-regulating the expression of EZH2

    doi: 10.7150/jca.66959

    Figure Lengend Snippet: MiR-506-3p suppresses OvCa tumor metastasis of an orthotopic mouse model of ovarian cancer. HeyA8 cells were injected intraperitoneally into a nu/nu mouse, and an aggressive HeyA8 clone generated from ascites was developed for subsequent use (HeyA8ip1). The mice were randomly divided into control miRNA+DOPC and miR-506-3p+DOPC groups after intraperitoneal injection of HeyA8ip1 cells. (A) In vivo imaging confirmed that miR-506/DOPC nanoliposomes can inhibit OvCa tumor metastasis. (B) Comparison of the incidence of metastases in various organs. (C) Evaluation of miR-506-3p expression in HeyA8-ip1 tumors from control and miR-506-3p treated mice by miRNA in situ hybridization. EZH2 IHC staining was performed on HeyA8-ip1 tumor samples from control and miR-506-3p treated mice. The expression of EZH2 was calculated as the IHC staining score.

    Article Snippet: According to the manufacturer's instructions, the miR-506-3p mimic or miR-ctrl was transfected into the OvCa cells at a final concentration of 20 nM with LipofectamineRNAiMAX (Invitrogen, USA).

    Techniques: Injection, Generated, In Vivo Imaging, Expressing, In Situ Hybridization, Immunohistochemistry

    Clinical cases confirm a negative correlation between miR-506-3p and EZH2 in OvCa. (A) The expression of miR-506-3p in 100 OvCa patients was evaluated by miRNA in situ hybridization. OvCa samples from 100 patients were immunohistochemically stained for EZH2. (B) The expression of EZH2 was calculated as the IHC staining score.

    Journal: Journal of Cancer

    Article Title: MicroRNA-506-3p inhibits ovarian cancer metastasis by down-regulating the expression of EZH2

    doi: 10.7150/jca.66959

    Figure Lengend Snippet: Clinical cases confirm a negative correlation between miR-506-3p and EZH2 in OvCa. (A) The expression of miR-506-3p in 100 OvCa patients was evaluated by miRNA in situ hybridization. OvCa samples from 100 patients were immunohistochemically stained for EZH2. (B) The expression of EZH2 was calculated as the IHC staining score.

    Article Snippet: According to the manufacturer's instructions, the miR-506-3p mimic or miR-ctrl was transfected into the OvCa cells at a final concentration of 20 nM with LipofectamineRNAiMAX (Invitrogen, USA).

    Techniques: Expressing, In Situ Hybridization, Staining, Immunohistochemistry